解放军医学杂志2011，Vol.36Issue(12)：1286-1290,5.
姜黄素对人结肠癌耐药细胞HCT-8/VCR蛋白质双向电泳图谱的影响
Effects of curcumin on 2-D electrophoresis profiles of proteins in drug-resistant HCT-8/VCR cells of human colon carcinoma
摘要
Abstract
Objective The present study investigates the difference in the protein expression profiles of drug-resistant HCT-8/VCR cells of human colon carcinomas with and without curcumin treatment Methods The drug-resistant HCT-8/VCR cells of human colon carcinomas were cultured with curcumin at a dosage of 25 μmol/L for 24 h. and those in the control group were treated with physiological saline. Then, the total proteins of both groups were extracted. Solid-phase pH-gradient-based 2-D gel electrophoresis (2-DE) was used to separate the total protein in the drug-resistant HCT-8/VCR cells of human colon carcinomas with and without curcumin treatment, and the gels were stained with silver. A GS-800 scanner was used to produce the gel images, and the differentially expressed proteins were identified and analyzed using the PDQuest 8.0 software program. Results The 2-DE HCT-8/VCR cell patterns were acquired with clear background, high resolution, and better reproduction. Furthermore, the average protein spots of the images with and without curcumin treatment were 1030+69 and 1070 + 96. respectively, hi the curcumin-treated group, 33 protein spots were found to be differentially expressed, 8 were upregulated, and 18 were downregulated. Moreover, only two protein spots were expressed in the curcumin-treated HCT-8/VCR cells, whereas five were found in the control group. Conclusions The 2-DE profiles of human colon carcinoma HCT-8/VCR cells with and without curcumin treatment were primarily established, and 33 differentially expressed protein spots were distinguished. Further studies on these differentially expressed protein spots may be useful in understanding the mechanism of curcumin in reversing multidrug-resistant colon carcinoma cells.关键词
蛋白质组学/电泳,凝胶,双向/多药耐药相关蛋白质类/姜黄素/结肠肿瘤Key words
proteomics/ electrophoresis,gel,two-dimensional/ multidrug resistance-associated proteins/ curcumin/ oolonic neoplasms分类
医药卫生引用本文复制引用
李雷,傅仲学,覃勇,卢伟东,王学虎,陈旺盛..姜黄素对人结肠癌耐药细胞HCT-8/VCR蛋白质双向电泳图谱的影响[J].解放军医学杂志,2011,36(12):1286-1290,5.基金项目
重庆市卫生局中医药重点项目(2009-1-2) （2009-1-2）
