解放军医学杂志2011，Vol.36Issue(12)：1298-1300,3.
+Gz暴露下高胆固醇兔胆囊排空功能、胆囊收缩素及生长抑素的变化
Effects of +Gz exposure on gallbladder emptying function, cholecystokinin, and somatostatin in rabbits with high cholesterol diets
摘要
Abstract
Objective The present study explores the effects of +Gz exposure on the gallbladder emptying function, cholecystokinin (CCK). and somatostatin (SS) in rabbits with high cholesterol diets and investigates its mechanism in the occurrence of cholecystolithiasis. Methods Twenty-four male New Zealand rabbits were randomly divided into the high cholesterol diet (control group, n=8) and high cholesterol diet plus +Gz exposure groups. The latter was divided into the four- and six-week +Gz exposure groups (n=8) based on the exposure time. Radioimmunoassay was used to determine the CCK and SS contents of the gallbladder at the end of the experiment in the fourth and sixth weeks and to calculate the gallbladder volume and maximum emptying ratio. A microcomputer biodynamic pressure monitor was used to record the hydrostatic pressure in the gallbladder to measure its capacity. Moreover, the bile properties and formation of concretion were observed with the naked eye, and polarized light microscopy was used to observe cholesterin crystallization on the gallbladder wall. Results The gallbladder capacity increased upon +Gz exposure for four and six weeks, indicating that the maximum emptying ratio (E%) decreased, the empty and residual volumes improved, and the pressure increased (P<0.05). After +Gz exposure for four and six weeks, the CCK contents in the experimental groups were evidently lower than that in the control group and gradually decreased (P<0.05) as the +Gz exposure time increased. On the other hand, after +Gz exposure for four and six weeks, the SS contents in the experimental groups were higher than that in the control group and gradually improved (P<0. 05) as the +Gz exposure time increased. After +Gz exposure for four and six weeks, bile was turbid and sticky with cholesterol crystals and without visible concretion. Conclusions Therefore, +Gz exposure may cause abnormal gallbladder emptying functions, decrease CCK content, increase SS content, and thus cause bile stasis. These results indicate that + Gz exposure provides the prerequisites for the occurrence of cholecystolithiasis.关键词
加速度/胆固醇/胆囊收缩素/生长抑素Key words
acceleration/ cholesterol/ cholecystokinin/ somatostatin分类
医药卫生引用本文复制引用
肖国丰,张洪义,何晓军,赵刚,孔亚林,刘洋..+Gz暴露下高胆固醇兔胆囊排空功能、胆囊收缩素及生长抑素的变化[J].解放军医学杂志,2011,36(12):1298-1300,3.基金项目
全军"十一五"科技攻关项目(08G047) （08G047）
