激光生物学报2011,Vol.20Issue(6):729-733,5.DOI:10.3969/j.issn.1007-7146.2011.06.004
紫外光诱导的油菜菌核病菌抗扑海因菌株的特性研究
Characteristics of the Resistant Mutants of Sclerotinia sclerotiorum to Iporodione by Ultraviolet Rays
摘要
Abstract
In the laboratory, by ultra-violet rays, the iprodione-resistant mutants of Sclerotinia sclerotiorum were induced, and the resistances of iprodione-resistant mutants were tested by rate of mycelia growth. The results showed that the EC50 values of iprodione of all iprodione-resistant mutants ranged from 0. 1159 μg/mL to 604. 2200 μg/mL, with the average of 117. 1363 μg/mL. The EC50 of UVIPrSS-7 , UVIPrSS-8 , UVIPrSS-15 , UVIPrSS-16, and UVIPrSS-18 were higher than 160 μg/mL. Sclerotial numbers and weights of the mutants, which cultured in PSA medium with iprodione of 1 μg/mL, were more than control with no iprodione. It suggested that in this experiment, the iprodione-resistant strains were easily obtained and there might be resistance risk of iprodione.关键词
油菜菌核病菌/扑海因/紫外光/抗药性Key words
Sclerotinia sclerotiorum/ iprodione/ ultraviolet rays/ fungicide-resistance分类
农业科技引用本文复制引用
陈方新,齐永霞,高智谋..紫外光诱导的油菜菌核病菌抗扑海因菌株的特性研究[J].激光生物学报,2011,20(6):729-733,5.基金项目
公益性行业(农业)科研专项(3-21) (农业)