激光生物学报2011，Vol.20Issue(6)：734-740,7.DOI:10.3969/j.issn.1007-7146.2011.06.005
弱磁场对骨骼肌肌质网钙调控作用的研究
Effects of Weak Magnetic Field on Ca2+ Modulation of Skeletal Muscle Sarcoplamic Reticulum
摘要
Abstract
Objective: Discuss the effects of weak magnetic field on isolated sarcoplasmic reticulum (SR) Ca2+ modulation, calcium pump (Ca2+-Mg2+-ATPase) and Ca2 + release channel (Ryanodine Receptor, RyR) activity, so as to explain the mechanism of biological effects caused by electromagnetic fields in terms of molecular level and signal transduction system. Methods;The Ca + modulation and Ca + -ATPase activity, NADH oxidation initial rates and superoxide production of SR exposed to 0. 4 mT weak magnetic field ( MF) were investigated with dynamic Ca2 * dye spectrum method, [3 H ] -Ryanodine binding assay was investigated by isotope Labeling. Results: Weak MF exposure decreased SR Ca + uptake and Ca2 + -ATPase activity obviously, increased SR Ca2 + release and [3 H ] -Rryanodine binding, up-regulated the initial rates of NADH oxidation and the production of superoxide. Conclusion: It is indicated that 0. 4 mT weak magnetic field exposure for 30 min inhibited Ca2 + -ATPase activity and promoted the RyR channel function.关键词
弱磁场/肌质网/钙调控/钙泵/钙释放通道Key words
weak magnetic field/ sarcoplasmic reticulum/ calcium modulation/ Ca2 + -ATPase/ ryanodine receptor分类
生物科学引用本文复制引用
刘仁臣,吴永刚,程和平,谢国秋,陆静,夏子奂..弱磁场对骨骼肌肌质网钙调控作用的研究[J].激光生物学报,2011,20(6):734-740,7.基金项目
安徽省自然科学基金项目(KJ 2010B213) （KJ 2010B213）
上海市重点科研项目基金(09JC1413800)资助课题 （09JC1413800）
