激光生物学报2011，Vol.20Issue(6)：778-781,4.DOI:10.3969/j.issn.1007-7146.2011.06.012
果蝇odd蛋白的原核表达及多克隆抗体的制备研究
A Study about the Prokaryotic Expression of Odd Fusion Protein and the Preparation of Its Polyclonal Antibody
摘要
Abstract
odd is a novel heart specific expression gene. Obtaining the odd protein and it' s antibody is the first step to study its function in heart development. The cDNA fragment of part coding sequence of odd was amplified by PCR and the obtained fragment was cloned into PET-28a vector. After identified by restriction enzyme and sequenced, the recombinant which containing odd gene was transformed into Escherichia coli BL21 strain and the fusion protein was induced by IPTG. A New Zealand white rabbit was immunized with the obtained protein which was purified by immobilized Glutathione Sepharose to prepare polyclonal antibody. The antibody was identified by Western blot. We had successfully obtained GST-tag fusion protein of odd and anti-odd polyclonal antibody with high sensitivity and specificity. It's lays a solid foundation for the further studies of odd function.关键词
odd/融合蛋白/原核表达/多克隆抗体Key words
odd/ fusion protein/ prokaryotic expression/ polyclonal antibody分类
生物科学引用本文复制引用
张义,吴秀山,邓云,叶湘漓,鲁建鑫,彭佩莹,赵阳,江志钢,李发祥,王跃群,袁婺洲..果蝇odd蛋白的原核表达及多克隆抗体的制备研究[J].激光生物学报,2011,20(6):778-781,4.基金项目
国家自然科学基金项目(81170088,30871340,81170229,30930054,30970425) （81170088,30871340,81170229,30930054,30970425）
湖南省普通高校学科带头人培养计划(湘教通2008-315) （湘教通2008-315）
国家精品课程(教高函2009-21) （教高函2009-21）
