江汉大学学报：自然科学版2011，Vol.39Issue(3)：82-85,4.
感受态细胞制备和质粒转化冰浴时间对大肠埃希菌转化效率的影响
Effects of Freezing Time During Preparation of Competent Cells and Plasmid Transformation on Transformation Efficiency of E.coli
摘要
Abstract
The affecting factors on transformation efficiency of E.coli were studied,which include plasmid DNA dosage putting into transforming system,freezing time during preparation of competent cells and plasmid transformation.These tests were used to analyse the best transforming parameters and optimize the methods of preparation of competent cells and plasmid transformation.The results indicated that the parameters of the highest transformation efficiency in this study were 1～10 ng of plasmid（pBR322） DNA dosage,30～60 min of freezing time during preparation of competent cells,as well as 30 min and 120 min of freezing time during plasmid transformation.Out of these conditions,it decreased the transformation efficiency of E.coli.The results of this study will provide technology basis for the transformation tests requiring high transformation efficiency.关键词
质粒/感受态细胞/转化效率/冰浴时间Key words
plasmid/competent cell/transformation efficiency/freezing time分类
生物科学引用本文复制引用
罗锋,余腾,范丽梅..感受态细胞制备和质粒转化冰浴时间对大肠埃希菌转化效率的影响[J].江汉大学学报：自然科学版,2011,39(3):82-85,4.基金项目
武汉市科技局青年科技晨光计划项目 （）
