经济动物学报2011，Vol.15Issue(3)：160-163,4.
蟾蜍壶菌病病原遗传分化研究
Genetic Differentiation of the Pathogen of Batrachochytrium dendrobatidis in Toads
摘要
Abstract
The chytridiomycosis is a significant emerging disease of amphibians in recent years which seriuosly does harm to the wild and breeding population. To ascertain the condition of the Batrachochytrium dendrobatidis infection history in the population of toad, the medicinal economic animal, and enhance the prevention for this disease, 32 toad specimen collected from Sichwan keeping in a museum were assayed by Taqman-MGB fluorescence probe quantitative polymerase chain reaction to detect Batrachochytrium dendrobatidis. Besides, the products of QPCR were cloned and sequenced to identify the origin of the pathogen by sequence alignment and phylogenetie analysis. Finally we got the standard curve： Y = - 3.0X ＋ 32.39 and the related coefficient： R2 = 0.999 6. Total 12 positive samples reported the detec- tion rate as 37.5 %. Meanwhile the phylogenetic analysis indicated that a certain extent differentiation of the chytrid fungi in our country exists. One kind of the tested strain showed highly genetic relationship with the strains from the North America, South America and Europe. Another one is obviously different from the strains found in the other areas of the world with special characteristics. It suggest that the pre- vention and treatment for chytridiomycosis of toad should be more targeted and should not copy foreign experience aimlessly.关键词
蟾蜍/馆藏标本/壶菌/实时PCR/系统发育分析Key words
toad/specimens collection/Batrachochytrium dendrobatidis/real-time PCR/phylogeneticanalysis分类
农业科技引用本文复制引用
曾朝辉,白世卓,朱蕴绮,王晓龙..蟾蜍壶菌病病原遗传分化研究[J].经济动物学报,2011,15(3):160-163,4.基金项目
北京市科学技术研究院“萌芽后续”人才培养计划资助项目 （）
