吉林农业大学学报2011，Vol.33Issue(6)：643-648,6.DOI:22-1100/S.20110928.1508.004
东北山樱根际细菌群落结构多样性分析
Analysis of the Bacterial Community Structures Diversity in Rhizosphere of Cerasus sachalinensis Kom
摘要
Abstract
The bacterial community structures of Cerasus sachalinensis Kom. In topsoil and rhizospheric soil in wild and cultivated conditions were studied by means of PCR - DGGE (denaturing gradient gel electrophoresis) , and their differences in the rhizosphere of Cerasus sachalinensis and other neighboring plant in the pristine growing conditions were also analyzed. Partial genes were amplified from soil bacteri al community DNA using 16S rDNA gene primers. The diversity of PCR-amplified products was detected by DGGE. The results show that the bacterial community diversities in Cerasus sachalinensis rhizospheric soil were always higher than in topsoil of both cultivated and wild conditions, and higher in the cultivated soil. In pristine growing conditions, the specific bacterial community structures were formed in Cerasus sachalinensis rhizosphere in the courses of plant growth and development. However, the rhizosphere bac terial community diversities of Cerasus sachalinensis were higher than those of the other neighboring plant and the control.关键词
东北山樱/细菌/群落结构/多样性Key words
Cerasus sachalinensis/ bacteria/ community structure/ diversity分类
农业科技引用本文复制引用
秦嗣军,吕德国,李志霞,刘灵芝,刘国成,马怀宇..东北山樱根际细菌群落结构多样性分析[J].吉林农业大学学报,2011,33(6):643-648,6.基金项目
国家自然科学基金项目(30871688 （）
30900967 （）
辽宁省教育厅项目(2008631) （2008631）
