吉林农业大学学报2011，Vol.33Issue(6)：682-686,5.DOI:22-1100/S.20110826.1534.001
旋毛虫T626-55基因减毒沙门氏菌疫苗的构建及其在小鼠体内表达
Construction and in vivo Expression of Trichinella spralis T626-55 Gene Vaccine Carried by Attenuated Salmonella
摘要
Abstract
Highly immunogenic gene T626-55 of Trichinella spiralis was amplified using PCR reaction and cloned into pcDNA3.1 eukaryotic expression plasmid. The recombinant plasmid was transformed into S. enterica Typhimurium phoP/phoQ strain by electroporation. In vivo expression of the target gene was testified. Mice were orally administrated with the recombinant bacteria and after 5 days of administration, spleen and Peyer's patch were aseptically removed for reverse transcription-polymerase chain reaction (RT - PCR) and indirect fluorescence assay (IFA) . The results show that Salmonella harboring T626-55 gene coding eukaryotic plasmid T626-55-pcDNA3.1-PQ was successfully constructed. The transcription of T626-5S gene was detected in spleen and pp, and expression of T626-55 protein was confirmed in spleen.关键词
旋毛虫/T626-55基因/沙门氏菌/反转录PCR/间接免疫荧光Key words
Trichinella spiralis/ T626-55 gene/ Salmonella/ RT-PCR/ IFA分类
农业科技引用本文复制引用
杜婧,刘攀,唐斌,高鹤,刘明远..旋毛虫T626-55基因减毒沙门氏菌疫苗的构建及其在小鼠体内表达[J].吉林农业大学学报,2011,33(6):682-686,5.基金项目
国家自然科学基金项目(NSFC30771885) （NSFC30771885）
国家杰出青年基金项目(NSFC30825033) （NSFC30825033）
