吉林农业大学学报2012，Vol.34Issue(1)：76-80,89,6.DOI:22-1100/S.20111102.1026.001
富含多糖多酚的侧柏叶片总RNA提取方法
Extraction of Total RNA from the Leaves of Platycladus orientalis Rich in Polysaccharides and Polyphenol
摘要
Abstract
The study compared the four methods of Trizol, CTAB, SDS-phenol, and improved SDS-phe-nol in extraction of total RN A from the leaves of Platycladus orientalis. The electrophoresis results showed that Trizol and CTAB method could not be successfully used for RNA isolation from Platycladus orientalis leaves. SDS-phenol method could be used for isolating RNA, but with abundant polysaccharides inside it did not satisfy the further experiments. Comparably, the improved SDS-phenol method could extract RNA from Platycladus orientalis leaves with higher quality through adding low concentration of ethanol and others . The further experiments displayed that the total RNA could be well transcribed and used for the specific amplification of gene fragments. It confirmed that the total RNA isolated by the improved SDS-phenol method could be well used for future gene cloning and expression studies.关键词
侧柏/RNA/多糖/多酚/改进的SDS酚法Key words
Platycladus orientalis/RNA/polysaccharide/polyphenol/improved SDS-phenol method分类
生物科学引用本文复制引用
王暑辉,徐倩,徐筱,徐吉臣..富含多糖多酚的侧柏叶片总RNA提取方法[J].吉林农业大学学报,2012,34(1):76-80,89,6.基金项目
林业公益性行业科研专项(201004009) （201004009）
