集美大学学报：自然科学版2011，Vol.16Issue(5)：346-351,6.
锯缘青蟹精氨酸激酶基因的克隆与表达
Cloning and Expression of Arginine Kinase from Mud Crab（Scylla serrata）
摘要
Abstract
The open reading frame gene sequences of Mud crab arginine kinase was cloned through the molecular biology technology method.The cloning of arginine kinase gene amplified with sequence analysis showed that the cloned DNA fragments had open reading frames of 1 071 bp,and predicted to encode proteins with 356 amino acid residues.The obtained gene was deposited in Genbank under accession number of GQ 851626.Sequence alignment revealed that the Mud crab arginine kinase shared 94 % of homology to Litopenaeus vannamei,and more than 90 % homology to other shellfishes,such as Carcinus maenas,Eriocheir sinensis.Mud crab arginine kinase gene was further recombined with the vector of pGEX-4T-3 and expressed in Escherichia coli BL21.The expressed protein revealed a band of about 65 ku on SDS-PAGE.Western blot analysis using anti-Mud crab AK polyclonal antibody revealed positive reaction to the GST-AK fusion protein.关键词
锯缘青蟹/精氨酸激酶/过敏原/cDNA克隆/重组表达Key words
Scylla serrata/arginine kinase/allergen/cDNA cloning/recombinant expression分类
生物科学引用本文复制引用
阮韦伟,沈苑,曹敏杰,苏文金,蔡秋风,刘光明..锯缘青蟹精氨酸激酶基因的克隆与表达[J].集美大学学报：自然科学版,2011,16(5):346-351,6.基金项目
国家自然科学基金资助项目 （）
福建省自然科学基金资助项目 （）
福建省科技计划重点资助项目 （）
福建省高校新世纪优秀人才计划资助项目 （）
集美大学创新团队基金资助项目 （）
李尚大集美大学学科建设基金资助项目 （）
