集美大学学报：自然科学版2012，Vol.17Issue(1)：26-32,7.
重组锰超氧化物歧化酶工程菌摇瓶发酵条件的优化
Optimization of Fermentation Conditions in a Shaker for the Recombinant Manganese Superoxide Dismutase from Engineering E. coli
摘要
Abstract
Fermentation media （carbon source, nitrogen source, inorganic salt） and cultivation condi- tions （inoeulum quantity, lactose concentration, time, temperature, shaker speed, pH value etc. ） by sin- gle factor researches were preliminarily optimized in a shaker for the recombinant manganese superoxide dis- mutase from engineering E. coli. The results showed that the optimized culture media for the engineering E. coliwere 1.5 % trytone, 1.5 % yeast extract, 1.0 % NaC1, 0.4 % KH2P04, 0.8 % K2HPO4, 1.5 % glycerine, 0. 2 % NH4C1 and 5 mmol/L MnC12. The optimized lactose induction conditions were growth for 5 h after inoculation at 5 % inoculum quantity, and then adding 0. 25 % lactose to induce for 6 h. The enzyme specific activity was the highest when the fermentation temperature was 37 ~E, the shaker speed was 180 r/min, and the original medium pH value was 7. 0. Under all of these optimized conditions, SOD from the engineering E. coli exhibited the enzymatic activity of 1 969. 9 U/mL and the specific activity of 1 081.8 U/mg.关键词
重组锰超氧化物歧化酶/发酵/乳糖/工程菌Key words
recombinant manganese superoxide dismutase/fermentation/lactose/engineering bacteria分类
生物科学引用本文复制引用
李鹤宾,刘光明,陈艳红,黄继福,朱艳冰..重组锰超氧化物歧化酶工程菌摇瓶发酵条件的优化[J].集美大学学报：自然科学版,2012,17(1):26-32,7.基金项目
福建省自然科学基金资助项目 （）
福建省教育厅科技项目 （）
厦门市科技计划项目 （）
厦门医学高等专科学校基金项目 （）
