解剖学杂志2011，Vol.34Issue(6)：739-742,4.
甲状旁腺素(1-34)对卵巢切除大鼠腰椎间盘退行性变的影响
Effect of parathyroid hormone (1-34) on lumbar intervertebral disc degeneration in ovariectomized rats
蔡金生 1张柳 1王文雅 2骆阳 3田发明 2程潭1
作者信息
- 1. 河北联合大学附属医院骨外科,唐山063000
- 2. 河北医科大学第三医院骨外科,石家庄050051
- 3. 河北联合大学病理学教研室,唐山063000
- 折叠
摘要
Abstract
Objective-. To investigate the effects and mechanism of parathyroid hormone (1-34) on lumbar intervertebral disc in ovariectomized rats. Methods-. 3-month-old female Sprague-Dawley rats were randomized into three groups. Ovariectomy was performed in OVX+NS group and OVX+PTH group, and a Sham surgery was performed in the Sham group. Four weeks after ovariectomy,the rats in OVX+PTH group and OVX+NS group received injection subcutaneously of 20 fig/kg PTHd-34) or saline respectively every week. The lumbar vertebra were harvested 20 weeks after ovariectomy and underwent bone mineral density (BMD) measurement, hematoxylin and eosin staining and histochemistry analysis. Results-. The bone mineral density of L2-3 vertebra in the OVX+NS group was significantly lower than that in the Sham group and OVX + PTH group; the histological score of L2-3 vertebra was significantly higher in OVX+NS group than that in the Sham group and OVX+PTH group. There was no significant difference in the immunostaining expressions of collagen I and collagen Ⅱ between the Sham group and OVX+PTH group. Conclusion-. Subcutaneous injection of parathyroid hormone (1-34) (20 μg ? Kg-1 ? Week-1) for twelve weeks can delay the degenerated process of intervertebral discs in ovariectomized rats.关键词
卵巢切除/椎间盘退变/免疫组织化学/甲状旁腺素Key words
ovariectomy/intervertebral disc degeneration/immunohistochemistry/parathyroid hormone (1-34)引用本文复制引用
蔡金生,张柳,王文雅,骆阳,田发明,程潭..甲状旁腺素(1-34)对卵巢切除大鼠腰椎间盘退行性变的影响[J].解剖学杂志,2011,34(6):739-742,4.
