解剖学杂志2011，Vol.34Issue(6)：747-750,4.
雌激素对大鼠胸腺细胞凋亡及Bcl-2、Bax表达的影响
Effects of estrogen on apoptosis and expression of Bcl-2 and Bax in rat thymus
摘要
Abstract
Objective-. To explore the effects of estrogen on the apoptosis and the expression of Bcl-2 and Bax in rat thymus. Methods-. The rats performed with ovariectomy were injected with estradiol benzoate. Thymus was obtained 7 days after the injection. Thymic indexes were measured. Apoptosis of the thymus was detected after Hochest 33342 staining and examined under an electron microscope. The expression of Bcl-2 and Bax in the thymus was detected with a immunohistochemical method. The expression of Bcl-2 mRNA and Bax mRNA in the thymus was detected by in situ hybridization. The test was taken in statistical treatment. Results: The thymus quality index in ovariectomy group was higher than that in the model control group. The thymus quality index of rats injected with estradiol benzoate was reduced respectively. Apoptotic cells and apoptotic bodies were found in the thymus in the groups treated with estradiol benzoate. The expression of Bcl-2 protein in rats injected with estradiol benzoate was lower than that in the control group, and Bax was higher. The expression of Bcl-2 mRNA and Bax mRNA in the thymus showed consistency with the expression of Bcl-2 and Bax. Conclusion: Estradiol benzoate may increase mass index of thymus, accelerate the degradation of the thymus, induce the apoptosis of the thymus, restrain the expression of Bcl-2 protein and promote the expression of Bax protein in the thymus of rats.关键词
雌激素/胸腺/细胞凋亡/Bcl-2/BaxKey words
estrogen/thymus/apoptosis/Bcl-2/Bax引用本文复制引用
李雅娜,孙研,崔春红,殷彦君..雌激素对大鼠胸腺细胞凋亡及Bcl-2、Bax表达的影响[J].解剖学杂志,2011,34(6):747-750,4.基金项目
山东省教育厅科技计划基金资助(J03K12) （J03K12）
