解剖学杂志2011，Vol.34Issue(6)：763-765,777,4.
卵巢癌细胞中OY-TES-1基因启动子CpG位点甲基化状态分析
CpG site methylation analysis of OY-TES-1 promoter in ovarian cancer cell
摘要
Abstract
Objective-. To investigate CpG site methylation of OY-TES-1 promoter in ovarian cancer cell line SKOV3. Methods-. OY-TES-1 expression in SKOV3 was detected by immunocytochemical staining with anti-OY-TES-1 antibody. The DNA fragment ( -127 bp'-hllO bp, including 24 CpG sites) of 5' flanking of OY-TES-1 gene was amplified from the ge-nomic DNA extracted from SKOV3 by bisulfite sequencing PCR (BSP). CpG site methylation of OY-TES-1 promoter was compared with testis. Results-. Positive expression of OY-TES-1 protein was found in SKOV3. Methylation percentage of 24 CpG sites on OY-TES-1 promoter tested was 80. 21% in SKOV3, which was higher than that in normal testis tissues. Conclusion-. OY-TES-1 has hypermethylated promoter CpG sites in SKOV3. Further study needs to learn the effect of these CpG sites methylation on the expression of OY-TES-1 when using DNA methyltransferase inhibitor 5-aza-2 -deoxycytidine (DAC).关键词
癌-睾丸抗原/卵巢癌/CpG/甲基化/OY-TES-1Key words
cancer-testis antigen/ovarian cancer/CpG/ methylation/OY-TES-1引用本文复制引用
范蓉,黄巍,郭文文,王炜炜,罗彬,宋慧,肖绍文,谢小薰..卵巢癌细胞中OY-TES-1基因启动子CpG位点甲基化状态分析[J].解剖学杂志,2011,34(6):763-765,777,4.基金项目
国家自然科学基金(30760055) （30760055）
广西高发疾病研究创新团队基金(桂教人[2007-59]号) （桂教人[2007-59]号）
广西中医学院课题(ZD200901 （）
P2009079) （）
