解剖学杂志2012，Vol.35Issue(1)：56-58,62,4.
凋亡相关蛋白在小鼠海马发育和老化中的表达
Expression of apoptosis related protein in the development and aging of mice hippocampus
邵姝元 1李潮 2张敬坤 3张莉3
作者信息
- 1. 辽宁医学院病理生理学教研室,锦州 121001
- 2. 辽宁医学院附属第一医院,锦州 121001
- 3. 辽宁医学院组织学与胚胎学教研室,锦州 121001
- 折叠
摘要
Abstract
Objective:To observe the changes of expression of Bcl-2 and Bax protein in the development and aging of C57/BL6 mouse hippocampus. Methods: Embyronic (E) 18, 20 day (d) and postnatal 1, 3, 5, 7, 14, 28 d and 2, 3, 6, 15, 18 month (M) mice were used in this study. Each group included 8 mice. Immunohistochemistry and immunoblot asssay were used to detect the expression of Bcl-2 and Bax protein in the mice hippocampus of different age. Results: From El6 d to P7 d, Bcl-2 and Bax immunoreactive cells increased in the hippocampus. On P14 d-P21 d, the two kinds of immunoreactive cells decreased, except the Bax positive cells in dentate gyrus which increased on P14 d while decreased on P21 d. Then this two kinds of cells remained unchanged after P28 d. Additionally, the average optical density of Bcl-2 and Bax on E18 d-P21 d in creased firstly and then decreased, except the Bcl-2 in dentate gyrus area. Immunoblotting analysis showed that the expres sion of Bcl-2 and Bax protein increased on E18 d-P7 d, decreased on P14 d-P21 d, and remained unchanged after P28 d. Bcl 2/Bax decreased on P1 d, increased on P3 d-P5 d, decreased on P7 d-P21 d, and remained unchanged on P28 d. Conclusion: The expression of Bcl-2 and Bax is high in the early stage of hippocampus development and then low in adult and senectitude. It may be related with the formation of the hippocampus.关键词
发育/海马/Bcl-2/BaxKey words
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邵姝元,李潮,张敬坤,张莉..凋亡相关蛋白在小鼠海马发育和老化中的表达[J].解剖学杂志,2012,35(1):56-58,62,4.
