解剖学杂志2012，Vol.35Issue(1)：72-74,3.
睾酮在自由基损伤的海马神经元保护作用中的机制
Mechanism underlying protective role of testosterone on primary cultured neuron damaged by free radical
蔡志平 1李朝晖 2石素琴 1崔慧先 3贾建新 4霍东升1
作者信息
- 1. 河北医科大学人体解剖学教研室,石家庄 050017
- 2. 包头医学院人体解剖学教研量,包头 014060
- 3. 河北医科大学第二医院神经外一科,石家庄 050017
- 4. 包头医学院第二附属医院药剂科,包头 014030
- 折叠
摘要
Abstract
Objective: To identify whether anti-apoptosis effect participate in the neuroprotection of testosterone. Methods: The hippocampal neurons of rats were primary cultured for 10 days, and then divided into a control group, H2O2 group and testosterone preconditioned followed by expose to H2 O2. The morphology of neurons were observed using fluorescence mi croscopy, and Bcl-2 and Bax expression in cultured hippocampal neurons were detected by Western blotting. Results: The apoptotic cells were found in H2O2 (100 μm) group exhibited as semilunar or broken nucleus with deep dyeing, and the processes were not clear. And, cultured neurons preconditioned with testosterone were shown with clear nucleus and nuclear fragments were hardly observed. Compared with the control neurons that shown the weak expression of Bcl-2 and Bax, H2 O2 led to a significant up-regulation in the expression of Bax and down-regulation in Bcl- 2 expression. The testosterone precondition statistically suppressed the overexpressed Bax and significantly recovered the down-regulation in Bcl-2 expression caused by H2O2. Conclusion:These findings suggest that neuroprotection of testosterone partially attribute to its capa bility to protect the free radical injured neurons against apoptosis.关键词
海马/神经元/凋亡/Bax/Bcl-2/Hoechst 33258/睾酮/自由基Key words
hippocampus/ neuron/ apoptosis/ Bax/ Bcl-2/ Hoechst 33258/ testosterone/ free radical引用本文复制引用
蔡志平,李朝晖,石素琴,崔慧先,贾建新,霍东升..睾酮在自由基损伤的海马神经元保护作用中的机制[J].解剖学杂志,2012,35(1):72-74,3.
