解剖学杂志2012，Vol.35Issue(1)：75-78,103,5.
胶原酶和自体血注入法建立脑出血动物模型的比较
Comparison of intracerebral hemorrhage animal models established by injecting collagenase and autologous blood
摘要
Abstract
Objective:To provide reference for study of pathologic mechanism and/or intervening therapy of intracerebral hemorrhage (ICH). Methods: Collagenase and autologous blood were injected into the caudate nucleus to establish ICH models in rats. The hematoma volume was measured by magnetic resonance imaging. The changes of brain water content (BWC) were measured by wet and dry weight methods. Evan's blue (EB) was used to measure the integrity of blood-brain bar rier (BBB). The changes of AQP4 expression were detected by immunofluorescence,and the contents of AQP4 were detected by West ern blotting. Results: The changes of hematoma volume, BWC, EB contents and AQP4 expression between the two ICH models showed a significant difference. Conclusion:Autologous blood animal model is relatively close to clinical ICH conditions.关键词
脑出血/动物模型/胶原酶/自体血/水通道蛋白4/大鼠Key words
intracerebral hemorrhage/ animal model/ collagenase/ autologous blood/ aquaporin 4/ rat引用本文复制引用
张波,孙善全,邱国平,甘胜伟,朱淑娟,钟小燕,伍修宇..胶原酶和自体血注入法建立脑出血动物模型的比较[J].解剖学杂志,2012,35(1):75-78,103,5.基金项目
国家自然科学基金(30470608) （30470608）
