江苏大学学报（医学版）2012，Vol.22Issue(1)：51-54,4.
急性胰腺炎患者外周血单个核细胞p38MAPK信号通路的变化
Character of p38 MAPK signal pathway in peripheral blood mononuclear cells of patients with acute pancreatitis
李桂芬 1郭继强2
作者信息
- 1. 新乡医学院第一附属医院急诊科
- 2. 新乡医学院免疫学教研室,河南,新乡,453100
- 折叠
摘要
Abstract
Objective: To investigate the relationship between p38 MAPK ( or phosphorylation-p38 MAPK ) levels in peripheral blood mononuclear cell ( PBMC ) and disease severity in patients with acute pancreatitis ( AP ). Methods: Twenty-three patients with severe acute pancreatitis ( SAP ), 29 patients with mild acute pancreatitis ( MAP ), and 21 healthy volunteers were evaluated in this study. Levels of p38 MAPK mRNA in PBMC were measured by real-time PCR. Protein levels of p38 MAPK and P-p38 MAPK were detected by Western Blot. The data obtained were subjected to one-way ANOVA. Results: Levels of p38 MAPK mRNA in SAP group were increased by 11. 7% ( P <0. 05 ) compared with control group, while there were no difference between MAP and control groups. Levels of p38 MAPK protein in SAP group were increased by 18. 7% ( P < 0. 05 ) compared with control group, while there were no difference between MAP and control groups. Phosphorylation levels of p38 MAPK in SAP group were significantly increased by 444. 1% ( P<0. 01 ) contrast to control group, and increased by 44. 1%( P <0. 05 )compared with MAP group. That in MAP group was elevated by 27. 8%( P <0. 05 )compared with control group. Conclusion: It was phosphorylation levels but not total levels of p38 MAPK that significantly increased in PBMC from a-cute pancreatitis, and phosphorylation-p38 MAPK took a close relationship with the grade severity of AP.关键词
丝裂原活化蛋白激酶p38/磷酸化丝裂原活化蛋白激酶p38/急性胰腺炎/外周血单个核细胞Key words
p38 MAPK/ phosphorylation- p38 MAPK/ acute pancreatitis/ peripheral blood mononuclear cell分类
医药卫生引用本文复制引用
李桂芬,郭继强..急性胰腺炎患者外周血单个核细胞p38MAPK信号通路的变化[J].江苏大学学报（医学版）,2012,22(1):51-54,4.
