吉首大学学报：自然科学版Issue(5)：75-79,5.
Pseudomonas koreensis JDM-2株ACC脱氨酶基因的克隆和表达
Cloning of ACC Deaminase Gene from Pseudomonas koreensis JDM-2 and Expression in Escherichia Coli BL21
摘要
Abstract
The acdS gene encoding an ACC deaminase was amplified by PCR from genomic DNA of Pseudomonas koreensis JDM-2,and the PCR product was ligated into the pMD18-T vector and then sequenced. The acdS gene was excised from plasmid pMD18ACDS by double digestion with BanH Ⅰ and Hind Ⅲ ,and cloned into the prokaryotic expression vector pQE30 to provide plasmid of pQE30ACDS. The ACC deaminase was expressed in Escherichia coli BL21 harboring the plasmid pQE30ACDS by IPTG induction. The sequence analyses showed that the acdS gene was 1 017 bp in length,DNA homolo- gy of the acdS genes between the Pseudomonas koreensis JDM-2 and the previously reported different bacterial strains in GenBank was 86% to 99 %. The SDS-PAGE analyses showed a single protein band with a molecular weight of 39 kDa expressed in Escherichia coli BL21 ,and its enzyme activity was 0. 214 U/mg.关键词
Pseudomonas/koreensis/JDM-2/acdS基因/ACC脱氨酶/表达Key words
Pseudomonas koreensis JDM-2/acd S gene/ACC deaminase/expression分类
生物科学引用本文复制引用
龚凤娟,张宇凤,刘丹丹,恩特马克·布拉提白,吾鲁木汗·那孜尔别克..Pseudomonas koreensis JDM-2株ACC脱氨酶基因的克隆和表达[J].吉首大学学报：自然科学版,2011,(5):75-79,5.基金项目
国家自然科学基金资助项目 （）
吉首大学研究生科研创新项目 （）
