净水技术2011，Vol.30Issue(6)：62-65,4.
固相萃取-高效液相色谱法检测饮用水中痕量微囊藻毒素
Determination of Trace Microcystins in Drinking Water by Solid Phase Extraction-High Performance Liquid Chromatography (SPE-HPLC) Method
摘要
Abstract
To develop a method for determination of trace microcystin (MC) in drinking water by solid phase extraction-high performance liquid chromatography (HPLC) methods was introduced. MCs in water were enriched and purified by solid phase extraction, eluated by methanol, and the eluate was concentrated by N2 gas. The HPLC separation was carried out with column using Vmethanol: Vphosphate buffer solution =45:55 as mobile phase, which was detected under wavelength of 238 nm. The results show that a good linearity on range of 0～1 μg/L can be achieved and average recovery rate are 100.9 % and 92 %, respectively. The method is sensitive and accurate for the determination of MCs in water.关键词
痕量微囊藻毒素/固相萃取(SPE)/高效液相色谱(HPLC)/饮用水Key words
trace microcystine/solid-phase extraction (SPE)/ high performance liquid chromatography (HPLC)/ drinking water分类
建筑与水利引用本文复制引用
马颖,王莹,刘涛利,张建柱..固相萃取-高效液相色谱法检测饮用水中痕量微囊藻毒素[J].净水技术,2011,30(6):62-65,4.
