实用临床医药杂志2011，Vol.15Issue(24)：46-49,4.
无血清悬浮法筛选人结肠癌干细胞样亚群的初步研究
Preliminary study of the serum- free suspension method for screening human colon cancer stem cell- like subsets
摘要
Abstract
Objective To explore the application of serum - free medium (SFM) suspension to culture human colon cancer cells, screen and identify human colon cancer cells in tumor stem cells subsets, and give relevant intervention. Methods Fresh colon tumor tissues were taken and put in pri mary culture. They were then isolated from the primary colon cancer cells to form tumor stem cell ball in SFM and grow adherently in serum - free medium ( SSM). 5 - Fu was used to intervene cancer stem cells ball which were obtained on the screening. Ratio of side population cells (SP) , ability of cell colony formation, expression of normal intestinal stem cell marker Musashi - 1 and expression of colon cancer stem cell - specific marker CD]33 were examined. Results Human colon cancer cells obtained from primary culture could survive, proliferate and form cancer stem cell ball in SFM. SP cells in cancer stem cells ball were high, and they had high rate of clone formation. Mushashi - 1 and CD133 expression were higher, and the inhibition 5 - Fu on SP cells was low. After 5 - Fu intervention, the proportion of SP cells in the colon cancer stem cells ball, the expressions of Musashi - 1 and CD133 in creased. There was no significant difference in cloning formation. Conclusion A very small amount of cancer stem cells ball can be isolated from human colon cancer cells by SFM training method, and 5 - Fu intervention can re - enriched cell subsets in the tumor stem cells.关键词
结肠癌/肿瘤干细胞/Musashi -1/CD133Key words
colon cancer/ cancer stem cells/ Musashi - 1/ CD133分类
医药卫生引用本文复制引用
苏沐,张全安..无血清悬浮法筛选人结肠癌干细胞样亚群的初步研究[J].实用临床医药杂志,2011,15(24):46-49,4.基金项目
中国高校医学期刊临床专项资金(41120011) （41120011）
