江苏大学学报（自然科学版）2012，Vol.33Issue(1)：102-105,4.DOI:10.3969/j.issn.1671-7775.2012.01.021
超滤膜浓缩重组α-乙酰乳酸脱羧酶及酶法清洗
Concentration recombinant of α- acetolactate decarboxylase with ultrafiltration membrane and enzymatic cleaning
摘要
Abstract
a-Acetolactate decarboxylase was concentrated from Bacillus subtilis with polyethersulfone ( PES) ultrafiltration membrane. The effects of temperature and pH value on membrane flux were investigated to determine the recovery conditions of chemical and enzymatic cleaning of ultrafiltration membrane. The result show that membrane flux is affected strongly by pH value of enzyme solution. After 10-fold concentration, the permeate flux at pH of 5. 1 is only 20. 3% of that at pH of 7. 1. The recovery of a-acetolactate decarboxylase can reach 89. 6% at the optimum ultrafiltration conditions for pressure of 0. 1 Mpa, temperature of 35 ℃ , and pH value of 7.1. During enzymatic cleaning, the high neutral protease activity of 1. 25 × 10~4 U · L~(-1) can result in membrane refouling. The best cleaning effect can be achieved by combining neutral protease of 1. 25 × 10~4 U · L~(-1) and sodium hypochlorite of 1% with flux recovery rate of 97.0%.关键词
超滤膜/膜通量/α-乙酰乳酸脱羧酶/酶法清洗/中性蛋白酶Key words
ultrafiltration membrane/ permeate flux/ a-acetolactate decarboxylase/ enzymatic cleaning/ neutral protease分类
化学化工引用本文复制引用
蒙健宗,齐向辉,李丛,吴华德,农翠菲..超滤膜浓缩重组α-乙酰乳酸脱羧酶及酶法清洗[J].江苏大学学报（自然科学版）,2012,33(1):102-105,4.基金项目
国家自然科学基金资助项目(21006041) （21006041）
广西科学研究与技术开发计划项目(桂科产10100021-2) （桂科产10100021-2）
江苏省博士后基金资助项目(0901012B) （0901012B）
江苏大学高级专业人才科研基金资助项目(08JDG009) （08JDG009）
