扬州大学学报（农业与生命科学版）2011，Vol.32Issue(4)：1-6,6.
抗菌肽Folicidin-3在大肠杆菌中融合表达及抑菌活性
Biological characteristics of the antibacterial peptide Fowlicidin-3 fusion expressed in Escherichia coli
摘要
Abstract
Based on the amino acids of Fowlicidin-3 and the preference condon of Escherichia coli, a 117 bp Fowlicidin-3a gene and a 102 bp Fowlicidin-3b gene were designed and synthesized. The two genes were cloned to the-express vector to construct recombinant plasmids pET-32a-C-Fowlicidin-3a and pET-32a-Tev-C-Fowlicidin-3b. Then recombinant plas-mids were transferred into E. Coli BL21 Rosetta-2 and induced by IPTG. The fusion proteins Trx-Fowlicidin-3a and Trx-Fowlicidin-3b were expressed and purified. The results showed that Fowlicidin-Sa and Fowlicidin-3b were effectively obtained by cleavage of the fusion protein with enterokinase and Tev proteases. Antimicrobial activity assays demonstrated that the Fowlicidin-3a and Fowlicidin-3b had candidacidal activities. The minimal inhibitory concentration of Fowlicidin-3a and Fowlicidin-3b was 2 μg · mL-1.关键词
Fowlicidin-3/抗菌肽/融合表达/抑菌试验Key words
Fowlicidin-3/ antibacterial peptide/ fusion expressions/ bacteriostasis experiment分类
医药卫生引用本文复制引用
和祯泉,刘天健,吕英军,鲍恩东..抗菌肽Folicidin-3在大肠杆菌中融合表达及抑菌活性[J].扬州大学学报（农业与生命科学版）,2011,32(4):1-6,6.基金项目
国家高技术研究发展计划项目(863-2007AA100602) （863-2007AA100602）
天津市科技支撑计划重点项目(81) （81）
