扬州大学学报（农业与生命科学版）2011，Vol.32Issue(4)：23-27,38,6.
红壤连作花生不同生育期根际微生物区系变化研究
Studies on microbial population dynamics in rhizospheres of the continous cropping peanuts in red soil at different growth periods
摘要
Abstract
The microbial population dynamics in the rhizospheres of continous cropping peanuts was studied by the plate counting method and DGGE. The results showed that the numbers of bacteria, actinomycete and fungus significantly increased in peanut continuous cropping soil with long-term fertilization. The numbers of bacteria and actinomycete in M (pig manure) and BM (pig manure with effective microorganisms) were significantly higher than those in F(chemical fertilizer) , but the numbers of fungus were less in M and BM than in F. PCR-DGGE analysis indicated that the microbial biodiversity decreased in continuous different fertilization treatments, the dominant species was Sphin-gomonas sp, and the number of R. Solanacearum increased. The Shannon Index and the Richness of M and BM were higer than F, Compared with F, BM could improve the microbial community diversity in the rhizospheres of continous cropping peanuts. Soil microflora unbalance and increasing soil borne-desease pathogens were two main factors of the peanut continuous cropping dbstacle in red soil.关键词
红壤/连作花生/生育期/根际微生物区系/DGGEKey words
red soil/ peanut continous cropping/ development stage/ rhizosphere microbe/ DGGE分类
农业科技引用本文复制引用
王小兵,骆永明,刘五星,李振高..红壤连作花生不同生育期根际微生物区系变化研究[J].扬州大学学报（农业与生命科学版）,2011,32(4):23-27,38,6.基金项目
国家自然科学基金重点资助项目(40432005) （40432005）
中国科学院知识创新工程重要方向项目(KSCX2-YW-G-053-3) （KSCX2-YW-G-053-3）
江苏省科技支撑计划项目(BE2011360) （BE2011360）
