扬州大学学报（农业与生命科学版）2011，Vol.32Issue(4)：64-69,6.
恶疫霉侵染的草莓SSH文库的构建及分析
Construction and analysis of SSH library of strawberry crown upon infection with Phytophthora cactorum
摘要
Abstract
Strawberry crown rot caused by Phytophthora cactorum is one of destructive diseases endangering strawber- . Ry production worldwide. To explore the molecular mechanisms underlying the interaction of strawberry with P. Cactorum, a susceptible strawberry variety FDP821 to P. Cactorum was used as main material/in the study. With.mRNA from strawberry crowns infected by P. Cactorum as the tester and mRNA from the control crowns as the driver, a subtracted cDNA library of FDP821 was constructed using suppression subtractive hybridization.. Colony PCR on randomly picked clones showed that the inserts were between 300 - 1 000 bp. One hundred and nine clones were sequenced and ninety-two were identified as strawberry expressed sequence tags, representing 50 strawberry geries. BLASTX analysis demonstrated that these genes were involved in disease resistance, antioxidative stress, metabolism and protein synthesis. This study provides a data basis for understanding the molecular mechanisms of strawberry resistance to crown rot caused by P. Cactorum.关键词
草莓冠腐病/恶疫霉/抑制性差减杂交技术/表达序列标签Key words
strawberry crown rot/ Phytophthora cactorum/ suppression subtractive hybridization/ expressed sequence tags分类
农业科技引用本文复制引用
陈孝仁,王振荣,徐敬友,童蕴慧,May Bente Brurberg..恶疫霉侵染的草莓SSH文库的构建及分析[J].扬州大学学报（农业与生命科学版）,2011,32(4):64-69,6.基金项目
国家自然科学基金资助项目(31101395) （31101395）
江苏省基础研究计划项目(BK2011443) （BK2011443）
