扬州大学学报（农业与生命科学版）2011，Vol.32Issue(4)：86-89,4.
高效液相色谱法测定豆芽中2,4-二氯苯氧乙酸残留
Determination of 2,4-dichlorophenoxyacetic acid residues in bean sprout samples by high performance liquid chromatography
摘要
Abstract
A method for determination of 2,4-dichlorophenoxyacetic acid residues in bean sprout samples by solid phase extraction-high performance liquid chromatography was developed. 2,4-dichlorophenoxyacetic acid was extracted with alkaline water from bean sprout samples and then the total extract was cleaned up by the LC-18 cartridges and eluted by CH3OH after centrifugation, filtration and acidification. CH3OH-H2O CV/V, 4 : 1) was applied as mobile phase, the flow rate was 0. 8 Ml · min-1 , detection wavelength was 290 nm and the analysis was carried out by separation with Agilent TC-C18 column and determination with PDA detector. Under the experimental conditions the results of determination were as follows: the detection limit of 2,4-dichlorophenoxyacetic acid was 0. 01 mg · kg-1 (S/N=3) , the average fortified recoveries of 2,4-dichlorophenoxyacetic acid in the blank was 91. 16% - 108. 35% and the relative standard deviations (RSD, n = 5) was 3. 51% -6. 27%. The method was simple, sensitive, accurate and repeatable and can be used to determine 2,4-dichlorophenoxyacetic acid residues in bean sprout samples.关键词
豆芽/2,4-二氯苯氧乙酸/高效液相色谱/残留Key words
bean sprout/ 2,4-dichlorophenoxyacetic acid/ high performance liquid chromatography/ residues分类
化学化工引用本文复制引用
夏慧,陆自强,杨益众..高效液相色谱法测定豆芽中2,4-二氯苯氧乙酸残留[J].扬州大学学报（农业与生命科学版）,2011,32(4):86-89,4.基金项目
江苏省出入境检验检疫局科研项目(2009KJ32) （2009KJ32）
扬州市科技攻关项目(YZ2010079) （YZ2010079）
