军事医学2012，Vol.36Issue(2)：115-119,5.
利用免疫蛋白质组学鉴定炭疽杆菌蛋白MetK及其免疫原性验证
Immunogenic protein MetK of Bacillus anthracis identified by immunoproteomics
摘要
Abstract
Objective To confirm the immunogenicity of Bacillus anthracis MetK identified by immunoproteomics study. Methods Immunoproteomics study of B. Anthracis A16R revealed a protein MetK. metK genes were amplified by PCR and cloned into prokaryotic expression vector pET32a. Recombinant plasmid pET32a-merff was transformed into E. Coli BL21 ( DE3 ). MetK was expressed by inducing IPTG and purified by Ni affinity chromatography. Polyclonal antibody in mice was prepared by intraperitoneal injection. Western blot was used to assay the immunogenicity of MetK protein. Results and Conclusion MetK protein was successfully expressed and polyclonal antibody was produced. The polyclonal antisera can be used to study the function of MetK protein.关键词
免疫蛋白质组学/metK/芽孢杆菌/炭疽/基因表达/多克隆抗体Key words
immunoproteomics/ metK/ Bacillus anthracis/ gene expression/ polyclonal antibody分类
医药卫生引用本文复制引用
童超,刘先凯,任静晓,王东澍,高志奇,冯尔玲,朱力,廖祥儒,王恒樑..利用免疫蛋白质组学鉴定炭疽杆菌蛋白MetK及其免疫原性验证[J].军事医学,2012,36(2):115-119,5.基金项目
国家自然科学基金资助项目(81071322,81125012) （81071322,81125012）
