军事医学2012，Vol.36Issue(2)：128-132,5.
凝血酶体外促进肺癌细胞Glc-82周期进程和增殖及其分子机制
Effect of thrombin on cell cycle and proliferation of lung cancer cell Glc-82 in vitro and its molecular mechanism
许芝山 1朱凌云 2王秀冬 2赵云峰 1于爱平2
作者信息
- 1. 曲阜师范大学生命科学学院,山东曲阜,273165
- 2. 军事医学科学院放射与辐射医学研究所,北京,100850
- 折叠
摘要
Abstract
Objective To evaluate the effect of thrombin on cell cycle and proliferation of lung cancer cells Glc-82 in vitro and the mechanism. Methods The effect of thrombin on Glc-82 cells proliferation was determined by CCK8 assay. For cell cycle and cell apoptosis analysis, the cells were stained with propidium iodide ( PI) or Annexin-V/PI and monitored on a flow cytometer. Real-time fluorescent quantitation polymerase chain reaction was used to detect the level of phos-phatase and tensin homologue deleted on chromatosome 10( PTEN ) mRNA. The protein expression level of PTEN and AKT phosphorylation was detected by Western blotting assay. Results Thrombin (0.5 and 1. 0 U/ml ) significantly promoted Glc-82 cell proliferation ( P <0. 01 ). Thrombin ( 0. 5 U/ml ) facilitated the cell cycle process from G, phase to S phase. Thrombin markedly down-regulated the expression level of PTEN, and increased AKT phosphorylation. Thrombin showed no effect on Glc-82 cells apoptosis. Conclusion Thrombin promotes lung cancer Glc-82 cell cycle progression and proliferation, which may be regulated by PTEN/PDK/AKT signaling pathway.关键词
凝血酶/细胞增殖/细胞周期/细胞凋亡/PTENKey words
thrombin/ cell proliferation/ cell cycle/ apoptosis/ PTEN分类
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许芝山,朱凌云,王秀冬,赵云峰,于爱平..凝血酶体外促进肺癌细胞Glc-82周期进程和增殖及其分子机制[J].军事医学,2012,36(2):128-132,5.
