军事医学2012，Vol.36Issue(3)：196-198,216,4.
多西他赛热敏脂质体的制备及含量和包封率的测定
Preparation of docetaxel-loaded thermosensitive liposomes and determination of its content and entrapment efficiency
摘要
Abstract
Objective To prepare docetaxel( DTX )-loaded thermosensitive liposomes ( DTL ) and establish a method for determination of the content and entrapment efficiency. Methods DTLs were prepared by the thin film hydration method. HPLC was used to determine drugs in DTL. The entrapment efficiency of DTL was determined, followed by high-speed centrifugation at 13 400 × g for 15 min. Results DTLs appeared uniform and the mean particle size was 104 nm. The transition temperature ( Tm ) of DTL was 42. 20 ℃. The excipients and solvents did not interfere with the assay. A good linear relationship was found between the peak area and the concentration of DTX in the range of 1. 0 - 100. 0 μg/ml ( r = 1 ). The average recovery was 99. 50% ( RSD = 0. 65% ). The entrapment efficiency of DTL exceeded 98% . Conclusion The thin fllm hydration method can be used for DTL preparation. The method is simple, accurate, reliable, sensitive and applicable for the determination of the content and entrapment efficiency of DTL.关键词
多西他赛/脂质体/抗肿瘤药/热敏/HPLC/含量测定/包封率Key words
docetaxel/liposomes/antineoplastic agents/thermosensitivity/HPLC/content determiration/entrapment efficiency分类
医药卫生引用本文复制引用
付盟,龚伟,张慧,梅兴国..多西他赛热敏脂质体的制备及含量和包封率的测定[J].军事医学,2012,36(3):196-198,216,4.基金项目
国家"973"计划资助项目(2009CB30305) （2009CB30305）
国家重大新药创制科技重大专项资助项目(2009ZX09301-002) （2009ZX09301-002）
