江西农业学报2011，Vol.23Issue(11)：132-134,3.
24株剑麻根际联合固氮菌的16S rDNA序列分析
16S rDNA Sequence Analysis of 24 Strains of Associative Nitrogen Fixation Bacteria from Rhizosphere of Sisal
摘要
Abstract
Through the separation and purification of associative nitrogen fixation bacteria, as well as the determination of their nitrogenase activities by using acetylene reduction method, twenty -four bacterial strains with nitrogen fixation activity were obtained from the rhizosphere of sisal plants, which were planted in the main production area of Hainan, Cuangxi and Guangdong. Their nitrogenase activities varied from 5.8742 to 1765.6590 nmol/( mL · H). The results of sequence analysis of 16S rDNA showed that the amplification section of 16S rDNA from the tested strains had no large changes in the length, and it was about 1500 bp. The 24 strains belonged to 6 different genera, through searching their homologic sequences in the GenBank and constructing their phylogenetic tree.关键词
剑麻/根际/联合固氮茵/16S rDNAKey words
Sisal/ Rhizosphere/ Associative nitrogen fixation bacteria/ 16S rDNA分类
农业科技引用本文复制引用
张世清,李庆洋,高建明,陈河龙,郑金龙,刘巧莲,易克贤..24株剑麻根际联合固氮菌的16S rDNA序列分析[J].江西农业学报,2011,23(11):132-134,3.基金项目
中央级公益性科研院所基本科研业务费专项资金(TTBB110210) （TTBB110210）
国家麻类现代产业技术体系建设专项(CARS-19-E17) （CARS-19-E17）
引进国外技术管理人才项目(20114600031). （20114600031）
