军医进修学院学报2011，Vol.32Issue(12)：1204-1206,1223,4.DOI:11-3275/R.20111024.1515.003
131I近距离放射对C6胶质瘤细胞杀伤的实验研究
Killing effect of 131I brachytherapy on C6 brain glioma cells:An experimental study
邰军利 1李安民2
作者信息
- 1. 北京市健宫医院神经外科,北京100054
- 2. 解放军总医院海南分院神经外科,海南三亚572013
- 折叠
摘要
Abstract
Objective To study the effective dose and effective dose rate of killing the cultured C6 glioma cells for a novel 131I liquid-filled brachytherapy applicator in order to direct the clinical treatment of glioma. Methods Those cultured C6 glioma cells in the tissue culture flasks, placed at distance of 5mm and 30mm respectively from a novel 60mCi 131I-filled radiotherapy cyst, were detected in the aspects of its apoptosis and survival rates every 4 days(72h each time) by cloning efficiency and flow cytometry with Annexin V-FITC/PI double staining. Results Apoptosis occurred in some C6 glioma cells 72h after exposed to 60mCi 131I, with an apoptotic rate of 16.42% ± 1.66%. Death was observed in proliferating C6 glioma cells. The survival rate of C6 glioma cells decreased 72h after exposed to 60mCi 131I with a minimal survival rate of 59.0% ± 2.39%. Apoptosis of C6 glioma cells occurred when the dose of 131I brachytherapy was <400mGy/h. The effective dose of 131I brachytherapy for killing C6 glioma cells was 47.64-61.65mGy/h. Apoptosis of C6 glioma cells decreased with the increasing dose of 131I brachytherapy. The effective absorption dose for inducing apoptosis of C6 glioma cells was 47.64-61.65mGy/h with an accumulative absorption dose of ≥ 3.9Gy 72h after exposed to 60mCi 131I. Conclusion A radiotherapy cyst can induce apoptosis of some C6 glioma cells 72h after exposed to 60mCi 131I and decrease the survival rate of C6 glioma cells. The effective dose of 131I radiotherapy for C6 glioma cells is ≥ 47.64-61.65mGy/h and its 72h accumulative dose is 3.9Gy.关键词
131I/放疗囊/C6胶质瘤细胞/凋亡Key words
131I/radiotherapy cyst/C6 glioma cell/apoptosis分类
临床医学引用本文复制引用
邰军利,李安民..131I近距离放射对C6胶质瘤细胞杀伤的实验研究[J].军医进修学院学报,2011,32(12):1204-1206,1223,4.
