军医进修学院学报2011，Vol.32Issue(12)：1253-1256,4.DOI:11-3275/R.20110809.1555.001
异丙酚对缺血再灌注损伤神经细胞的保护作用
Role of propofol in protection of neurons against ischemia/reperfusion injury
摘要
Abstract
Objective To study the role of propofol in protection of neurons against ischemia/reperfusion (I/R) injury. Methods Hippocampal cells of Wistar rats at the age of 12h were cultured in vitro and a hypoxia and reoxygenation model of primary hippocampal cells was established. Morphology of hippocampal cells was observed. Cell survival rate was calculated with trypan blue staining. An in vivo model of I/R injury was established. The rats were divided into middle cerebral artery occlusion(MCAO) model group, propofol treatment group, and normal control group. Longa's neurological symptoms were scored. Cerebral infarction size was measured with TTC and H&E staining. Results The survived hippocampal cells were characterized by a large number of cytodendrites with a dense neuron net in hypoxia and reoxygenation model group after propofol treatment. The cell survival rate was significantly higher in propofol(100μmol/L) treatment group than in MCAO model group(56.2 ± 3.7 vs 38.6 ± 4.3, P<0.05). The score of neurological symptoms was significantly higher in 24h MCAO model group than in propofol treatment group (0.8 ± 0.5 vs 2.3 ± 0.5, P<0.05). TTC and H&E staining indicated that the infarction size was significantly smaller in propofol treatment group than in MCAO model group (123.21 ± 11.04 vs 214.95 ± 16.79, P<0.05). H&E staining showed more normal cells in propofol treatment group than in MCAO model group. Conclusion Propofol can protect neurons against I/R injury.关键词
异丙酚/缺血再灌注损伤/海马神经元Key words
propofol/ischemia/reperfusion injury/hippocampal neurons分类
医药卫生引用本文复制引用
陈娜,周建平,黄安宁,袁京,许秀丽,冯泽国..异丙酚对缺血再灌注损伤神经细胞的保护作用[J].军医进修学院学报,2011,32(12):1253-1256,4.基金项目
"十一五"国家科技重大专项"重大新药创制"(2009ZX09103-722) （2009ZX09103-722）
第45批中国博士后科学基金特别资助 （）
