军医进修学院学报2011，Vol.32Issue(12)：1271-1273,3.DOI:11-3275/R.20110630.1624.002
低温冷冻对皮肤高分子量角蛋白表达的影响
Effect of cryopreservation on expression of high molecular weight epidermal keratin
蔡宏 1董宁 2姚咏明 2李绍然 2贾晓明2
作者信息
- 1. 空军总医院皮肤科,北京100036
- 2. 解放军总医院第一附属医院,北京100037
- 折叠
摘要
Abstract
Objective To study the changes in cryo-preserved high molecular weight epidermal keratin and analyze the effect of cryobiology on epidermal cytoskeleton. Methods Adult human skin samples were divided into fresh group, 4℃. Group, -20°C group, -80℃ group and -196℃ group. Changes in epidermal keratin were observed under an electron microscope with immunohistochemical staining 14 days after cryopreservation. Keratin level in skin tissue was analyzed with an image analyzer. Results The expression level of keratin decreased with the decreasing preservation temperature in 4℃ group, -20℃ group, and -80℃ group, and was similar in -196℃ group and fresh group. Conclusion Hypothermia injury of skin is closely correlated with cytoskeleton, and keratin plays an important role in the mechanism of cryobiology.关键词
皮肤/高分子量角蛋白/低温Key words
skin/high molecular weight/hypothermia分类
医药卫生引用本文复制引用
蔡宏,董宁,姚咏明,李绍然,贾晓明..低温冷冻对皮肤高分子量角蛋白表达的影响[J].军医进修学院学报,2011,32(12):1271-1273,3.
