军医进修学院学报2012，Vol.33Issue(1)：63-65,3.DOI:11-3275/R.20110815.0910.002
NDLIP对大鼠脑缺血再灌注损伤后血脑屏障的保护作用
Effect of noninvasive delayed limbischemic preconditioning on endothelial barrier antigen expression and blood-brain barrier permeability after ischemia/reperfusion injury in rats
摘要
Abstract
Objective To observe the protective effect of noninvasive delayed limbischemic preconditioning(NDLIP) on blood brain barrier(BBB) after ischemia/reperfusion(I/R) injury in rats. Methods Thirty male SD rats were randomly divided into NDLIP group, I/R group, and sham operation group, 10 in each group. A focal cerebral I/R injury model of rats was established by occluding the middle cerebral artery (MCA) for 2h and reperfusing for 24h. Rats in NDLIP group were preconditioned before ischemia by occluding the lateral femoral arteries 3 times at an interval of 5min and reperfusing for 5min, once a day for 3 days. The prefrontal cortex was removed from rats 24h after reperfusion and cut into sections after freezing. Rats in I/R and sham operation groups were not given NDLIP. Expressions of endothelial barrier antigen(EBA) and fibrinogen were detected with immunohistology staining and infarct volume was measured. Results The expression of EBA was 11.61 ± 0.98, 5.3 ± 1.24 and 15.2 ± 1.02, respectively, in NDLIP, I/R and sham operation groups(P<0.01). The expression of EBA was 11.61 ±0.98, 8.60 ±3.12 and 1.30 ±0.46, respectively, in NDLIP, I/R and sham operation groups(P<0.01). The expression of Fibrinogen was 5.56 ± 2.58, 8.60 ± 3.12 and 1.30 ±0.46, respectively, in NDLIP, I/R and sham operation groups (P<0.01). The infraction volume was (218.05 ± 112.14)mm3 and (250.68 ± 129.76)mm\ respectively, in NDLIP and I/R groups(P<0.01). Conclusion NDLIP can significantly increase the EBA expression, decrease the BBB permeability and cerebral infarction volume after I/R injury, thus protecting BBB against I/R injury.关键词
无创性肢体缺血预适应/血脑屏障/缺血再灌注损伤/内皮屏障抗原Key words
noninvasive delayed limbischemic preconditioning/ blood-brain barrier/ reperfusion-injury/ endothelial barrier antigen分类
医药卫生引用本文复制引用
刘式威,刘维会,滕长波,刘政华,吕霞,杨桂蓉,刘志刚..NDLIP对大鼠脑缺血再灌注损伤后血脑屏障的保护作用[J].军医进修学院学报,2012,33(1):63-65,3.基金项目
国家"973"重点基础研究发展计划资助项目(2004CB720302) （2004CB720302）
