军医进修学院学报2012，Vol.33Issue(1)：82-83,87,3.DOI:11-3275/R.20110810.1443.001
鼠降钙素基因相关肽两种不同检测方法比较
Two different methods for detection of calcitonin gene-related peptide in rats: A comparative study
张凯 1郝秀华 1张金英 1邓子辉 1王录焕 1薛辉 1颜光涛1
作者信息
- 1. 解放军总医院基础医学研究所生化研究室,北京100853
- 折叠
摘要
Abstract
Objective To evaluate the efficiency of radioimmunoassay (RIA) and enzyme linked immunoassay (ELIS A) in detecting calcitonin gene-related peptide(CGRP) in rats. Methods CGRP levels in standard substance and quality control of CGRP, 100 plasma samples from normal rats and middle cerebral artery occlusion (MCAO) model of rats were measured by RIA and ELISA, respectively, and their precision rates were compared. Results The relative coefficient of CGRP in normal rats and MCAO model of rats detected by RIA and ELISA was 0.986 and 0.962, respectively(P<0.01). Conclusion The CGRP detected by RIA is consistent with that detected by ELISA, thus both RIA and ELISA can be used in detection of CGRP.关键词
鼠降钙素基因相关肽/放射免疫分析/酶联免疫分析Key words
calcitonin gene related peptide/ radioimmunoassay/ enzyme linked immunoassay分类
医药卫生引用本文复制引用
张凯,郝秀华,张金英,邓子辉,王录焕,薛辉,颜光涛..鼠降钙素基因相关肽两种不同检测方法比较[J].军医进修学院学报,2012,33(1):82-83,87,3.
