昆虫学报2011，Vol.54Issue(11)：1281-1287,7.
球孢白僵菌和金龟子绿僵菌不同菌株对黑足角胸叶甲成虫的致病力评价
Pathogenicity evaluation of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against adults of Basilepta melanopus ( Coleoptera: Eumolpidae)
摘要
Abstract
In order to screen entomopathogenic fungi strains with high virulence against the new pest Basilepta melanopus on Camellia oleifera, the growth rate and sporulation of different Beauveria bassiana and Metarhizium anisopliae strains were investigated, and bioassay evaluation of B. Bassiana and M. Anisopliae against B. Melanopus adults was carried out through dipping in spore suspension. The results showed that the growth rate and sporulation of different fungi strains were significantly different. The four strains, I. E., MaYTTR-04, BbFZ-17, MaZPTR-01 and BbTK-01, had higher growth rate and sporulation than other strains. The cumulative mortality of B. Melanopus adults was gradually increased with time after inoculation with B. Bassiana and M. Anisopliae. The cumulative mortality of B. Melanopus adults reached 100% at 7 d after inoculation with B. Bassiana, while the mortality of adults at 14 d after inoculation with MaZPTR-01 and MaYTTR-04 were 80. 3% and 78. 8% , respectively. The cadaver rate of adults inoculated with B. Bassiana was significantly higher than that inoculated with M. Anisopliae. The cadaver rates of BbTK-01 and BbFZ-17 were highest, being 85.7% and 75. 8% , respectively. The median lethal time (LT50) values of B. Bassianu strains BbXJ-01, BbFZ-17 and BbTK-01 were shortest, being 3.0, 3. 3 and 3. 4 d, respectively. However, the LTXvalues of the two M. Anisopliae strains, MaZPTR-01 and MaYTTR-04, were 6.0 d and 6.2 d, respectively. The results of bioassay showed that B. Bassiana strains had higher virulence to B. Melanopus adults than M. Anisopliae, especially the two strains, BbTK-01 and BbFZ-17, had higher lethality and infection rate than other strains, and at the same time, they had the characteristics of rapid growth and high sporulation, suggesting that the two strains have great potential in biocontrol of B. Melanopus adults.关键词
球孢白僵菌/金龟子绿僵菌/生物学特性/黑足角胸叶甲/致死中时( LT50)/僵虫率Key words
Beauveria bassiana/ Metarkizium anisopliae/ biological characteristics/ Basilepta melanopus/ median lethal time ( LT50 ) / cadaver rate分类
数理科学引用本文复制引用
何学友,蔡守平,童应华,熊瑜,黄勇,谢家冬,陈顺立..球孢白僵菌和金龟子绿僵菌不同菌株对黑足角胸叶甲成虫的致病力评价[J].昆虫学报,2011,54(11):1281-1287,7.基金项目
福建省科技厅农业科技重点项目(2011N0011) （2011N0011）
福建省林业厅科研项目(闽林科2009[ 8]) （闽林科2009[ 8]）
科技部农业科技成果转化资金项目(2010GB2C400215) （2010GB2C400215）
福建省森林培育与林产品加工重点实验室资助项目 （）
