昆虫学报2012，Vol.55Issue(1)：91-100,10.
丝带凤蝶线粒体基因组全序列及其系统学意义
The complete mitochondrial genome of the dragon swallowtail,Sericinus montela Gray ( Lepidoptera: Papilionidae) and its phylogenetic implication
摘要
Abstract
The phylogenetic relationship between the zerynthiids and the other groups of Papilionidae has long been a controversial issue.In this study,the complete nucleotide sequences of the mitochondrial genome (mitogenome) of Sericinus montela belonging to the zerynthiids was determined using long PCR and conserved primer walking approaches.Meanwhile,the phylogenetic analysis of this species with other representative papilionid species was conducted to clarify their phylogenetic relationships on the mitogenomic level.The results showed that the entire mitochondrial DNA (mtDNA) molecule is 15 242bp in length with the content of A,T,G and C of 40.1%,40.8%,7.4% and 11.7%,respectively,and its nucleotide composition of the genome is highly A + T biased (80.8%) ; all the 13 protein coding genes (PCGs) use standard initiation codons ATN,and all the PCGs use common stop codon (TAA),except for the ND4 and ND4L genes,which terminate with a single T; all tRNA genes form a typical clover-leaf secondary structure,except for the tRNAser ( AGN),whose DHU arm forms a simple loop ;there are twelve intergenic spacer regions ranging from 2 to 65 bp in size,and fifteen overlaps ranging from 1 to 8 bp in size in the mitogenome sequence.The neighbor joining and maximum parsimony phylogenetic analyses based on the 13 PCG sequences showed that Sericins montela and Luehdorfia chinensis form a clade that is sister to the Parnassius bremeri,suggesting that they should be designated as a taxon of tribal level within the subfamily Parnassiinae in the family of Papilionidae.关键词
鳞翅目/凤蝶科/丝带凤蝶/线粒体基因组/系统发育分析Key words
Lepidoptera/Papilionidae/Sericinus montela/mitochondrial genome/phylogenetic analysis分类
生物科学引用本文复制引用
季刘伟,郝家胜,王莹,黄敦元,赵金良,朱朝东..丝带凤蝶线粒体基因组全序列及其系统学意义[J].昆虫学报,2012,55(1):91-100,10.基金项目
国家自然科学基金项目(41172004) （41172004）
安徽省高校省级自然科学研究重点项目(KJ2010A142) （KJ2010A142）
安徽省优秀青年科技基金(08040106811) （08040106811）
中国科学院动物研究所动物进化与系统学院级重点实验室专项基金(0529YX5105) （0529YX5105）
