昆明医学院学报2011，Vol.32Issue(11)：31-35,5.
复方光明胶囊对实验性大鼠视网膜光损伤的作用
Effect of Compound Light Capsule on Rats' Retinal Light Injury Induced by Operation Microscope, an Electron Microscope Observation
摘要
Abstract
Objective To establish the rat model of retinal light injury, and observe the effect of Compound Light Capsule on the rat retinal light injury under electron microscope. Methods 30 SD rats (60 eyes) were divided into high dose group, middle dose group, low dose group, sham group, and normal group in random, 6 SD rats (12 eyes) in each group. Rats were intragastrically administered with Compound Light Capsule 7 days before modeling, and the administration lasted for 2 weeks. Experimental rats in each group were exposured to the light away from the corner (150 ± 3) mm except the normal group. The illumination of eyes in the horizontal position of operation microscope was ( 22 000 ± 1 000) lux, and continue light exposure for 30 minutes. The administration for another 1 week after exposure to the light. The retinal morphology changes of each group were observed under electron microscope. Results Normal rats had clear layers of retinal structures, the inner and outer segments of rod cells were in a neat and orderly arrangement. The inner and outer nuclear layer arranged in sequence and stained evenly. Each group was observed a thinner and sparser change in the outer nuclear layer after light exposure. The outer segments of rod cells arranged disorderly, part of the membranous discs were ruptured. Swollen mitochondria of the rod cells in inner segments were also observed. Outer nuclear layer cells showed karyopycnosis and maldistribution. The changes in each dose group were improved compared with sham group. Conclusion The establishment of the retinal light injury model is successful by using the operating microscope. Each dose of Compound light Capsules shows a protective effect on retinal light injury.关键词
复方光明胶囊/视网膜光损伤/透射电镜Key words
Compound light capsule/ Retinal light injury/ Electron microscope分类
医药卫生引用本文复制引用
彭华,马珊,王维绮,卜文超,董玉,马素红..复方光明胶囊对实验性大鼠视网膜光损伤的作用[J].昆明医学院学报,2011,32(11):31-35,5.基金项目
云南省科技厅科学研究基金资助项目(2009ZC153M) （2009ZC153M）
云南省教育厅科学研究基金资助项目(09Y0237) （09Y0237）
