昆明医学院学报2012，Vol.33Issue(1)：12-15,4.
染色体核型和bcr/abl融合基因表达与慢性髓细胞白血病急变的关系
The Relation of Karyotype and bcr/abl Fusion Gene with the Blast Crisis of Chronic Myeloid Leukemia
摘要
Abstract
Objective To explore the relation of chronic myeloid leukemia cytogenetic characteristics and disease development. Methods Direct short-term culture gbanding was used to detect the of karyotype of bone marrow chromosome in CML patients. Two- color fluorescence in situ hybridization was used to detect the bcr/abl fusion genes in CML patients, t test was used to statistically analyze the data. Results In the 264 cases of CML patients with bone marrow chromosomal testing, Ph negative chromosome was detected in 14 cases, the negative rate was 5.3%, the remaining cases were Ph positive chromosome and variation of ph chromosome, the positive rate was 94.7%. In 43 CML patients in blast crisis period, standard Ph chromosome was found in 22 cases, Ph negative chromosome in 9 cases, double Ph chromosome in 2 cases, the variation of Ph chromosome in 3 cases, standard Ph chromosome with other dasypyrum in 3 cases, Ph negative chromosome with other dasypyrum in 1 case, Ph negative chromosome with inversion in 2 cases, Ph positive chromosome with inversion in 1 case. Karyotype and fusion gene change occurred in 24 of 43 patients in blast crisis, and 19 cases had no change in the karyotypical chromosmes and the fusion gene. T test showed that there was significant differences in acceleration/blast crisis time (month) among patients CML patients with standard Ph chromosome, Ph negative chromosome and variation of Ph chromosome. (P＜0.01). Conclusions (1) Cloning evolution occurred in 55.8% of patients with chronic myeloid leukemia in acceleration/blast crisis period. (2) The accelevation/blast crisis period of CML patients with Ph negative, Ph variant and complex chromsome was shorter than that of CML patients with t [(9;22) (qll;34) ]. G) The CML cellheredity not only provide a reliable evidence for the diagnosis, and also plays an important role in the prognosis of CML.关键词
慢性髓性白血病/染色体核型/bcr/abl融合基因Key words
Chronic myelogenous leukemia/ Karyotype/ Bcr/abl fusion gene分类
医药卫生引用本文复制引用
李正发,邓明倩,沈晓梅,朱宝生,唐新华,史克倩,章印红..染色体核型和bcr/abl融合基因表达与慢性髓细胞白血病急变的关系[J].昆明医学院学报,2012,33(1):12-15,4.基金项目
云南省自然科学基金资助项目(94C038Q （）
云南省人民医院科研基金资助项目,2003YK011) （）
