兰州大学学报（医学版）2011，Vol.37Issue(4)：28-32,5.
高效毛细管电泳分离和测定丹红注射液中水溶性活性成分
Separation and determination of water-soluble active components in Danhong injection by capillary zone electrophoresis
摘要
Abstract
Objective To explore the separation and determination of water-soluble active components in Danhong injection by high performance capillary electrophoresis analysis technique. Methods 50 mmol/L phosphate buffer, pH 7.2, working voltage at 15 kV, temperature at 25 °C were used. Capillary electrophoresis was performed using a uncoated fused-silica capillary whose length is 48.5 cm and effective column length is 40 cm. Diode array detection was set at 210 nm. Water-soluble active ingredients, procatechuic aldehyde, Danshensu and procatechuic acid in Danhong injection electrophoresis were analyzed under the optimization of the electrophoretic separation conditions. The linear of those three compounds, detection limits and reproducibility of the method were studied. Results Under the optimizing conditions, procatechuic aldehyde, Danshensu and procatechuic acid in Danhong injection could be successfully determined within 11 min. Conclusion High performance capillary electrophoresis method is suitable for quantitative analysis of Danhong injection.关键词
高效毛细管电泳/丹红注射液/水溶性活性成分Key words
high performance capillary electrophoresis/ Danhong injection/ water-soluble active components分类
医药卫生引用本文复制引用
李倩倩,袁红燕,王利涛,甘晓辉,乔成栋..高效毛细管电泳分离和测定丹红注射液中水溶性活性成分[J].兰州大学学报（医学版）,2011,37(4):28-32,5.基金项目
中国科学院科技支新基金(2011-2013) （2011-2013）
兰州市科技专项计划基金(2010-1-156) （2010-1-156）
