临床口腔医学杂志2011，Vol.27Issue(12)：721-724,4.DOI:10.3969/j.issn.1003-1634.2011.12.006
大鼠涎腺放射损伤模型的建立及NBS1的表达变化
Expression and significance of NBS1 in irradiation injured salivary gland in rats.
摘要
Abstract
Objective: To investigate the expression of NBS1 genes in the radiation-injured model of salivary gland in rats, and preliminary explore the irradiated injury repair mechanism of NBS1 in salivary gland. Method: The rats were randomly divided into two groups, the irradiated rats(n=40) were fractionated exposed to a dose of 3Cy 6OC0 7-rays, given two days time >leading to an calculated dose of 3.6.9.12.15GY; The sham-irradiated controls(n=40) were anesthetized in parallel with the irradiated rats but not irradiated. After irradiation in two to four hours .the rats were sacrificed, the histomorphological changes were observed by HE staining; the ultrastructural changes were observed by transmission electron microscopy; RT-PCR were used to detect the NBSlexpression. Result:The organization were damaged even more serious in parotid gland than in submandibular gland; Compared with the controls, with the groups of dose above 9Gy in parotid gland and the dose above 12GY in the submandibular gland.the expression of NBS1 mRNA were reduced (P < 0.05).Conclusions:The model of irradiated injury salivary gland was successfully constructed. NBS1 might be involved in salivary gland irradiated injury repair.关键词
NBS1/MRN(MRE1 1-RAD50-NBS1)复合物/DNA双链断裂(DSB)/涎腺上皮细胞/放射性损伤修复Key words
NBS1/MRN complex/ DSB/ salivary gland epithelial cells/ irradiated injury and repair分类
生物科学引用本文复制引用
林丹,王代友,杨亦萍,卿海云,曹阳,陈超梅,沈洁,欧健波..大鼠涎腺放射损伤模型的建立及NBS1的表达变化[J].临床口腔医学杂志,2011,27(12):721-724,4.基金项目
国家自然科学基金项目(30960420)广西医疗卫生重点科研课题(桂卫重2010082) （30960420）
