临床误诊误治2012，Vol.25Issue(2)：14-16,3.DOI:10.3969/j.issn.1002-3429.2012.02.008
大鼠心肌缺血再灌注损伤模型的制备及改进
Preparation and Improvement of Rat Model of Myocardial Ischemia Reperfusion Injury
摘要
Abstract
Objective To summarize and improve the method of making rat myocardial ischemia and reperfusion injury (MIRI) model. Methods 60 SPF degree adult male SD rats were randomly divided into three groups (20 in each). The three methods were used to manufacture the MIRI model; cutting off the fourth rib; cutting off the second, third and fourth ribs, pulling and fixing ligation line; if there was no need to cut off the ribs, the small breast expander and the pipettor spear were used instead of knot method. Results The success rate of the three methods were 45% , 50% and 70% , respectively with significant difference (P >0.05). Making of the three models needed (7.5 ± 1.2)min, (9.0 ±1.9)min and (5.9 ±0.8)min, respectively with significant difference (P<0. 05) , and methods three group compared with methods one, two group with statiatical difference (P<0. 01). The index of CK-MB after reperfusion(1215 ±223)was different form that during ischemia( 1665 ±313) (P <0. 05). Conclusion There is no need to cut off the ribs, the small breast expander and the pipettor spear instead of knot method without cutting off ribs is preferred in manufacturing MIRI model.关键词
大鼠/心肌再灌注损伤/模型,动物Key words
Rat/ Myocardial reperfusion injury/ Model, animal分类
医药卫生引用本文复制引用
陈靖宜,刘菊英..大鼠心肌缺血再灌注损伤模型的制备及改进[J].临床误诊误治,2012,25(2):14-16,3.基金项目
国家自然科学基金资助课题(81171783) （81171783）
