临床与实验病理学杂志2012，Vol.28Issue(2)：184-188,5.
塞来昔布对鼻咽癌CNE-2Z细胞生物学行为的影响
Effects of celecoxib on the biologic behavior of nasopharyngeal carcinoma cell CNE-2Z
摘要
Abstract
Purpose To evaluate the effect of celecoxib ( a selective cyclooxygenase-2 inhibitor) on the proliferation, adhesion and invasion of human nasopharyngeal carcinoma ( NPC ) cell line CNE-2Z. Methods After treated with different concentrations of celecoxib for 24 hours, we used the colony formation assay to detect the effect of celecoxib on CNE-2Z cell proliferation, and the cell-cell adhesion assay, cell-matrix adhesion assay and migration assay to investigate the effects of celecoxib on adhesive and invasive capacity of CNE-2Z cell. Western blot and immunocytochemistry were used to detect the expression levels of COX-2. Results The colony formation assay showed that celecoxib could greatly inhibit CNE-2Z cell proliferation, the rate of colony formation was ( 85. 68 ± 1. 83 )% , ( 69. 00 ± 4. 08 )% , ( 59. 03 ± 2. 43 )% , ( 37. 28 ± 3. 25 )% and ( 12. 58 ± 3. 69 )% in 0, 25, 50, 75, and 100 μmol/L groups, respectively ( F = 321. 187, P < 0. 01 ). The cell-cell adhesion assay indicated that there was a significant difference between the experimental and control groups at the same time ( F = 321. 187, P < 0. 01 ). At 15 min time point, the cell adhesion rate was ( 12. 38 ± 1. 54 )% , ( 19. 30 ± 1. 49 )% and ( 20. 18 ± 1. 80 )% in 0, 25, 50 μmol/L group, respectively. At 45 min time point, there was ( 46. 34 ± 1. 37 )% , ( 58. 72 ± 1. 68 )% and ( 67. 15 ±0. 86 )% in 0, 25, 50 μmol/L group, respectively. The cell-matrix adhesion assay showed that the cell-matrix adhesion ability was significantly inhibited in 25, 50 |μmol/L group compared to the control group( t =24. 840, 38. 565 ,respectively, P <0. 01 ). The migration assay showed that there was a significant difference between the experimental and control groups( F = 36. 010, P < 0. 01 ), the cell numbers passing through PVPF were( 124. 3 ± 8. 02 ), ( 84. 3 ± 12. 22 ) and ( 55. 3 ±9. 29 ) in 0, 25, 50 μmol/L group, respectively. Western blot showed that COX-2 protein was significantly downregulated in25, 50 μmol/L group( t =25. 356, 73. 656, respectively, P <0. 01 ). Immunocytochemistry results showed that the expression levels of COX-2 were ( 380 ±11),(313 ±12 )and( 213 ± 8 ) in 0, 25, and 50 μmol/L groups, respectively, indicating a significant difference between groups ( F = 298. 578,P <0. 01 ). Conclusions Celecoxib can improve the cell-cell adhesion capacity, and inhibit the cell-matrix adhesion and migration capacity of CNE-2Z cell, which might be due to the inhibition of COX-2 expression.关键词
鼻咽肿瘤/塞来昔布/增殖/黏附/运动Key words
nasopharyngeal neoplasms/ celecoxib/ proliferation/ adhesion/ migration分类
医药卫生引用本文复制引用
罗伟仁,赵颖海,姜汉国,陈小毅..塞来昔布对鼻咽癌CNE-2Z细胞生物学行为的影响[J].临床与实验病理学杂志,2012,28(2):184-188,5.基金项目
广东省自然科学基金(031963)、广东医学院青年基金(XQ0608) （031963）
