临床与实验病理学杂志2012，Vol.28Issue(3)：278-281,286,5.
子宫颈病变组织中低分子量蛋白酶体表达与HPV16感染的关系
Association of the expression of the low molecular-weight protein with HPV16 infection in cervical lesions
摘要
Abstract
Purpose To explore the correlation between the mRNA and protein expression of the low molecular-weight protein with high risk HPV16 infection in cervical lesions. Methods Immunohistochemistry and RT-PCR was performed to detect the mRNA and protein expression levels of LMP2 and LMP7 from 152 cases Uighur women with cervicitis, cervical intraepithelial neoplasia( CIN ) and cervical squamous cell carcinoma. The HPV16 infection were detected by PCR. Results ( 1 )With the serious of cervical lesions,the protein expression of LMP2, LMP7 decreased progressively, and the mRNA expression levels were the same as protein expression. Down-regulation or loss expression of LMP2/LMP7 in CIN and cervical cancer were 25. 0%/15. 6% , 29. 7%/23. 4% , 17. 5%/34. 9% , 23. 8%/41. 3% respectively. LMP2 and LMP7 were significantly correlated with tumor grading and lymph node metastasis. ( 2 )PCR showed that the detection rate of HPV16 was increased with progress of cervical lesions, the HPV16 infective rate in cervicitis,CIN and cervical cancer was 8%( 2/25 ),67. 2%( 43/64 ),77. 8%( 49/63 ) respectively. And with the degree of malignant increasing its positive expression rate increased, positive expression differences between each group had a statistical significance. Down-regulation of LMP7 were associated with HPV16 infection in CIN( P < 0. 05 ), and down-regulation of LMP2 , LMP7 were associated with HPV16 infection in cervical cancer( P <0. 05 ). Conclusion Down-regulation in transcription expression level or loss protein expression of LMP was related to the progress of cervical cancer in Uighur women, and the HPV16 infection might be the most important cause.关键词
子宫颈肿瘤/低分子量蛋白酶体/HPV16Key words
cervical neoplasms/low molecular-weight protein/HPV16分类
医药卫生引用本文复制引用
哈提拉·吐尔逊,阿仙姑·哈斯木,阿布力孜·阿布杜拉,古力娜尔·库尔班..子宫颈病变组织中低分子量蛋白酶体表达与HPV16感染的关系[J].临床与实验病理学杂志,2012,28(3):278-281,286,5.基金项目
新疆维吾尔自治区自然科学基金(2009211B14) （2009211B14）
