摘要
Abstract
Purpose To investigate the expression of autophagy-related genes LC3,Beclin-l and apoptosis-related genes p53, BCL-2 in colorectal tumor tissues, and to explore their correlations with the tumorigenesis and development of colorectal carcinoma. Methods Tissue microarrays and immunohistochemistry were used to detect the expressions of LC3, Beclin-1, p53 and BCL-2 in 12 specimens of normal large intestinal mucosa, 29 specimens of large intestinal adenoma and 115 specimens of colorectal carcinoma. Results LC3 expression was significantly higher in colorectal carcinoma than those in large intestinal adenoma and normal large intestinal mucosa( P <0. 01 ). This high expression was associated with histological grade( P <0. 05 ). The expression of Beclin-1 was higher in adenoma and colorectal carcinoma than those in normal large intestinal mucosa( P <0. 01 ), but was not related to histological grade, Dukes stages or lymph metastasis( P >0. 05 ). The expression of p53 and BCL-2 increased gradually in normal large intestinal mucosa, adenoma and colorectal carcinoma( P<0. 01 ). The expressions of p53 and BCL-2 were correlated with Dukes stages and metastasis of lymph nodes( P < 0. 05 ). Beclin-1 expression was found to have positive correlation with p53 expression in colorectal carcinoma by the Spearman rank test( r =0. 224 3 , P <0. 05 ). Conclusion The increase of autophagic capacity and decrease of apoptosis are both involved in the carcinogenesis of colorectal carcinoma. The autophagy-related genes LC3, Beclin-1 and apoptosis-related genes p53, BCL-2 coordinate with each other, and may be related to tumorigenesis and the development of colorectal carcinoma. The union examination of LC3, Beclin-1, p53 and BCL-2 protein may be helpful for the prognosis judgment of colorectal carcinoma.关键词
大肠肿瘤/组织芯片/自噬/凋亡Key words
colorectal neoplasms/ tissue microarray/ autophagy/ apoptosis分类
医药卫生
