辽宁农业科学Issue(6):5-8,4.
铃兰组织培养及快速繁殖的研究
The Study of the Tissue Culture and Rapid Propagation of Convallaria Keiskei Miq
摘要
Abstract
In order to preserve the wild resources and meet the needs of germchits for planting, with method of tissue culture, root stems of Convallaria keiskei Miq were used as material to do the research on differentiation of root stems, rooting and rapid propagation of adventitious buds, transplantation of tube seedlings. The results show that MS +6-BA 0. 3 mg/L + NAA 0. 1 mg/Lwas suitable for growth and differentiation of root stems; the best medium for rooting of adventitious buds was White + NAA 0. 1 mg/L + IAA 0. 2 mg/L. The method of extend the time for rooting and add liquid medium to the culture flask for differentiation achieve the purpose of rapid propagation. The test tube seedlings from root stems maintained all biological characteristics of the wild Convallaria keiskei after transplanted.关键词
铃兰/组织培养/快速繁殖Key words
Convallaria keiskei Miq/ Tissue Culture/ Rapid Propagation引用本文复制引用
裴广盈,朱婷婷,陈悦,宁淑香,姜长阳..铃兰组织培养及快速繁殖的研究[J].辽宁农业科学,2011,(6):5-8,4.基金项目
辽宁省高等教育教学改革资助项目(20090304) (20090304)
辽宁师范大学教学改革资助项目(LSJG:20090108) (LSJG:20090108)