林业科学2011，Vol.47Issue(11)：37-43,7.
热激启动子控制的FT基因诱导杨树早期开花体系的优化
System Optimization of Precociously Flowering of Poplar Induced by FT Gene Controlled by a Heat Shock Promoter
摘要
Abstract
In order to achieve precociously flowering of poplar and shorten the genetic and breeding period, we studied factors affecting genetic transformation of FT1 gene controlled by Heat Shock Promoter in hybrid aspen clone ( Populus tremula × P. Tremuloides) and optimized the Agrobacterium-mediated transformation conditions. FT1 gene was transformed into hybrid aspen through optimized transformation protocol and the 2-3 months transgenic plants were successfully induced to precociously flower by heat shock. The results showed that regeneration frequency of kanamycin resistant plants of hybrid aspen were significantly affected by pre-culture of leaf disc, Agrobacterium concentration, incubation duration, and co-culture duration. Optimized transformation protocol was as follows: leaf discs were pre-cultured for 6 days on the callus-induction medium, incubated for 60 minutes in Agrobacterium solution to reach to the OD600 value of 0. 5 , and then co-cultured for 2 days. According to this protocol, regeneration frequency of kanamycin resistant plants can reach to 29. 84% . Expression of FT1 and precociously flowering of transgenic plants were induced by heat shock treatment of 37 ℃, 1 hour per day for 3 weeks. The size of transgenic plants was a limiting factor that affected the precociously flowering induced by heat shock, and plants with height less than 20 cm didn't show precociously flowering when induced by heat shock. Heat shock can induce more normal flowers, but extensive aberrance was still presented.关键词
FT基因/热激启动子(HSP)/杨树/遗传转化/早期开花Key words
FT gene/ heat shock promoter( HSP) / Populus/ genetic transformation/ precociously flowering分类
农业科技引用本文复制引用
贾小明,张焕玲,樊军锋..热激启动子控制的FT基因诱导杨树早期开花体系的优化[J].林业科学,2011,47(11):37-43,7.基金项目
陕西省"13115"重大科技专项(2009ZDKG-12). （2009ZDKG-12）
