林业科学2011，Vol.47Issue(11)：80-87,8.
脉冲磁场对葡萄座腔菌生长及侵染的影响
Effects of Pulsed Magnetic Field on Growth and Infestation of Botryosphaeria dothidea
摘要
Abstract
The effect of different treating time with the pulsed magnetic field (PMF) on the growth and infestation of Botryosphaeria dothidea was studied under a condition of fixed pulse rise time 10 ms. The results showed that short PMF treatment was able to effectively inhibit growth and infestation Botryosphaeria dolhidea, and the longer time the treatment , the stronger the inhibition. With pulse treatment for 45 min, colony was inhibited by 54. 11 % in 48 h, and the browning index of poplar callus inoculation after 72 h was only 26. 89% ; With pulse treatment for 60 min, the inhibitory effect was best, and the colony all was dead. The observations with SEM revealed that the colony had the characteristics of uneven thickness, the mycelium stuck together, the surface wrinkled, branch and parts of hyphae even dried out. Based on TEM, after pulse magnetic field treatment, the cell wall became significantly thinner and the inner parts disordered and degraded, moreover the cell structure was destroyed seriously and intracellular vacuoles increased. In the meantime, the poplar callus cells structure was influenced to different degrees by B. Dothidea, including deformation and disintegration of cell wall, decomposition of the layer, fracture of plasma membrane, plasmolysis; Cell organelles decomposed and deposited near cell walls, degradation of intracellular hyphae and cavum. The pulsed magnetic field had a strong inhibitory effect on the growth and infestation of B. Dothidea and could change the mycelium ulrastructure.关键词
脉冲磁场/葡萄座腔菌/致病力/菌丝/细胞结构Key words
pulsed magnetic field/ Botryosphaeria dothidea/ pathogenicity/ mycelium/ cell Structure分类
农业科技引用本文复制引用
张明,宋晓斌,张星耀,王振朝,梁军..脉冲磁场对葡萄座腔菌生长及侵染的影响[J].林业科学,2011,47(11):80-87,8.基金项目
国家自然科学基金项目(30671687). （30671687）
