棉花学报2011，Vol.23Issue(6)：545-551,7.
黄河流域棉花主要品种SSR指纹图谱构建及遗传差异分析
Construction of DNA Fingerprinting and Analysis of Genetic Diversity with SSR Markers for Maior Varieties from the Yellow River Vallev
摘要
Abstract
SSR markers were used to analyze the fingerprinting and genetic diversity of 23 major cultivars in the Yellow River Valley region during 2009-2010 and four varieties in Shandong provincial regional trial in 2010. Among 381 pairs of primers, 63 pairs with high polymorphisms and good repeatability were selected. Totally, 254 polymorphic bands were detected, with an average of 4.03 bands per primer. 23 varieties had unique bands by 22 primer pairs, which could be identified with one pair of specific primer except Lumianyan 21, Lumianyan 36, Lu 436 and Hanmian 559. All varieties could be distinguished by five primer combinations at least, and digital fingerprinting code was also established. UPGMA cluster analysis was used to estimate the genetic distances and to construct a dendrogram by NTSYS 2.1. The results showed that the coefficient of genetic similarity range was 0.5394-0.9685, with an average of 0.6878. Most of the varieties derived from the same province were clustered together, indicating their limitations on the geographic area.关键词
棉花/黄河流域/品种/SSR指纹图谱/遗传多样性Key words
cotton/ Yellow River Valley/ major varieties/ SSR fingerprinting/ genetic diversity分类
农业科技引用本文复制引用
韩宗福,王景会,申贵芳,王宗文,赵逢涛,李汝忠,张军,孔凡金,赵静..黄河流域棉花主要品种SSR指纹图谱构建及遗传差异分析[J].棉花学报,2011,23(6):545-551,7.基金项目
国家转基因生物重大专项(2008ZX08005-001) （2008ZX08005-001）
山东省农业良种工程(2010LZ005-02) （2010LZ005-02）
